protein A , cas number 91932-65-9 , ELISA or Immunohistochemical
Tests Antibody Test
Source:Protein A mutant of Staphylococcus aureus , expressed in E. Coli.
The recombinant Protein A is a genetically engineering protein
containing IgG-binding domains. Recombinant Protein A is ideal for
purification of polyclonal or monoclonal IgG antibodies. Protein A
binds to most human and mouse IgG subclasses (e.g., human IgG1,
IgG2, IgG4; mouse IgG2, IgG2a, IgG2b, IgG3). It also binds to cow,
guinea pig, hamster, house, pig and rabbit total IgG form.
Recombinant protein A can be coupled to solid separation medium
(such as agarose) for monoclonal and polyclonal antibody
purification. Recombinant protein A can be coupled to a variety of
molecules (such as fluorescent molecules, enzyme markers, biotin,
colloidal gold and radioactive markers). These coupled derivatives
can be used in antibody test in the process of Western-blot, ELISA
or immunohistochemical tests.
|Appearance||White or off white powder|
|Bioburden||≤ 1 cfu/ mg|
|SDS-PAGE||Single major band|
|SDS-PAGE (MW)||25000±1000 Daltons|
|UV Spectrum||E275/250 ≥ 1.2|
|E0.1% at 275nm = 0.17-0.23|
|Purity (HPLC)||≥ 95%|