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Legionella Pneumophila Elisa Test Kit , Human Elisa Kit Qualitative Immunoassays

Categories Elisa Test Kit
Brand Name: New Life
Model Number: 96 wells
Certification: ISO, CE
Place of Origin: China
MOQ: 10 kit
Payment Terms: T/T, Western Union, MoneyGram
Supply Ability: 100000kit/month
Delivery Time: 20-35days
Packaging Details: 96T/kit
Method: Elisa
Format: 96T/KIT
Valid: 24 month
Accuracy: 99.85%
Sensitivity: 99.84%
Application: Legionella pneumophila IgG/ IgM
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    Legionella Pneumophila Elisa Test Kit , Human Elisa Kit Qualitative Immunoassays

    Legionella pneumophila IgG/ IgM tests ELISA Test, Elisa Sandwich method, for quantitative measurement


    Product Name: Legionella pneumophila IgG/ IgM tests ELISA Test Kit


    Intended Use:


    The Legionella pneumophila 1-7 IgG and IgM tests are qualitative immunoassays for the demonstration of human antibodies directed against Legionella pneumophila serotypes 1 to 7. The assays are recommended to complement the findings of direct detection methods and for differential diagnosis in case of atypical pneumonia.

    Summary:

    Legionella belongs to the species of legionellaceae, genus Legionella. It is a gram negative, aerobic, pleomorphic, non-spore forming bacillus which is motile by means of a single or multiple polar or subpolar flagella. Currently, the genus includes 39 Legionella species. Immunologic diversity within species is reflected in the creation of serogroups. The most important human pathogen is the species Legionella pneumophila which comprises 14 serologic groups.

    Legionella are ubiquitous bacteria which reside in surface and drinking water and multiply in amoebae and other protozoa. Warm water systems with a temperature of 25-45°C are ideal for multiplication. After transmission to humans in aerosols, the bacteria are phagocytized by alveolar macrophages. So called evasion mechanisms enable bacteria not only to survive but also to multiply within a phagocytic cell.

    Due to non-specific clinical symptoms, diagnosis is based on laboratory techniques such as direct pathogen detection, derived antigen detection, or detection of specific antibodies.

    Direct pathogen detection in culture or in direct immunofluorescence assays (DFA) are of outstanding importance because fast diagnosis is often decisive for the patient. Sputum, tracheobronchial secretion, and pleura punctates are ideal samples. However, sensitivities of only 50-60 % are described for both techniques.

    To compensate for the above mentioned drawbacks of direct detection methods, serologic test systems are widely used. Since multiple serogroups of L. pneumophila are human pathogens, pool antigens are favored. In the SERION ELISA classic IgG/IgM, a mixture of serogroups 1-7 is bound to the solid phase

    Regents Provided


    ComponentAmount
    Plate: 96 wells configured in twelve 1X8-wellstrips coated with a
    formalin-inactivated sonicated preparation of L. pneumophila Groups 1-6 antigens.1 plate
    The strips are packaged in a strip holder and sealed in an envelope with desiccant.
    Conjugate: Conjugated(horseradish peroxidase) goatanti-humanIgG/IgA/IgM.15mL
    Ready to use. A white cap.
    Positive Control (Monkey Serum): A red cap.0.35mL
    Calibrator (Monkey Serum): A blue cap.0.5mL
    Negative Control (Human Serum): A green cap0.35mL
    Abnova (Sample Diluent): A green cap containing Tween-20, bovine serum
    albumin and phosphate-buffered-saline, (pH7.2±0.2). Ready to use. Note: Shake30mL
    Well Before Use.
    TMB: An amber cap containing 3,3’,5,5’-tetramethylbenzidine(TMB). Ready to use.15mL
    Contains OMSO<15%(w).
    Stop solution: Red cap containing 1M H2SO4, 0.7M HCl. Ready to use15mL
    Wash buffer concentrate (10X): dilute 1 part concentrate + 9 parts deionized or
    distilledwater.Adearcapcontaininga10Xconcentrated100mL
    phosphate-buffered-saline and solution (Blue solution). Note: 1*solution will have a
    pH of 7.2±0.2.

    Each kit contains the following components in sufficient quantities to perform the number of tests indicated on packaging label. Note: All reactive reagents contain sodium azide as a preservative at a concentration of 0.1% (w/v).


    The following components are not kit lot number dependent and may be used interchangeably with the ELISA assays: TMB, Stop Solution, and Wash Buffer.


    Note: Kit also contains:

    Component list containing lot specific information is inside the kit box. Package insert providing instructions for use.

    TEST PRINCIPLE


    The ELISA (Enzyme Linked Immunosorbent Assay) is an immunoassay, which is particularly suited to the determination of antibodies in the field of infectious serology. The reaction is based on the specific interaction of antibodies with their corresponding antigen. The test strips of the microtiter plate are coated with specific antigens of the pathogen of interest. If antibodies in the sample are present, they bind to the fixed antigen. A secondary antibody, which has been conjugated with the enzyme alkaline phosphatase, detects and binds to the immune complex. The colourless substrate pnitrophenylphosphate is then converted into the coloured product p-nitrophenol. The signal intensity of this reaction product is proportional to the concentration of the analyte in the sample and is measured photometrically.

    TEST PROCEDURE


    1. Remove the individual components from storage and allow them to warm to room temperature (20-25°C).


    2. Determine the number of microwells needed. Allow six Control/Calibrator determinations (one Blank, one Negative Control, three Calibrators and one Positive Control) per run. A Reagent Blank should be run on each assay. Check software and reader requirements for the correct Controls/Calibrator configurations. Return unused strips to the resealable pouch with desiccant, seal, and return to storage between 2 and 8


    3. Prepare a 1:21 dilution (e.g.: 10µL of serum + 200µL of Sample Diluent. NOTE: Shake Well Before Use) of the Negative Control, Calibrator, Positive Control, and each patient serum. The Sample Diluent will undergo a color change confirming that the specimen has been combined with the diluent.

    4. To individual wells, add 100µL of each diluted control, calibrator and sample. Ensure that the samples are properly mixed. Use a different pipette tip for each sample.


    5. Add 100µL of Sample Diluent to well A1 as a reagent blank. Check software and reader requirements for the correct reagent blank well configuration.


    6. Incubate the plate at room temperature (20-25°C) for 25± 5 minutes.


    7. Wash the microwell strips 5X.


    ORIENT NEW LIFE MEDICAL CO., LTD.
    Contact:Jerry Meng
    Email:Jerry @ newlifebiotest .com
    Tel.+86 18657312116
    SKYPEenetjerry
    Product Tags:

    elisa diagnostic kits

      

    rapid elisa test

      
    Quality Legionella Pneumophila Elisa Test Kit , Human Elisa Kit Qualitative Immunoassays for sale
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